Translational Science Biocore (TSB)

The mission of the UWCCC Translational Science BioCore (TSB) is to facilitate research by providing comprehensive services that enable cellular and molecular characterization of animal models and human derived biospecimens with deidentified clinical data. These services are provided through the combined expertise and equipment of the BioBank, the Translational Research Initiatives in Pathology (TRIP) laboratory, and the Experimental Animal Pathology Laboratory (EAPL).

The TSB focuses on support to researchers in  the following three critical areas:

  • Collection and distribution of patient-consented human-derived biospecimens with clinical data
  • Construction and distribution of disease-specific human tissue microarrays (TMAs), fully annotated with relevant clinical data
  • High quality services, expertise, and infrastructure in cellular, molecular, quantitative, and computational pathology

Faculty Leaders

Kristina matkowskyj – tsb faculty director, trip co-faculty leader, stephanie mcgregor – biobank faculty leader, william rehrauer – trip co-faculty leader.

uw trip lab

TSB - BioBank

Facility manager: everlyne nkadori, biobank lab contact:.

Phone: 608-262-8488 Hours: 7am-5pm M-F Email: [email protected]

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TSB - TRIP Lab

The TSB Translational Research Initiatives in Pathology (TRIP) laboratory provides specialized histological, histochemical, molecular and imaging analyses and consultative services to support the individual research needs of investigators within the UW campus and beyond. Select services include the construction and distribution of tissue microarrays; routine histology and complex immunohistochemistry and in situ hybridization; multispectral fluorescent and bright field imaging with quantitative morphometric analysis via Vectra and Nuance instruments; cell line authentication, PCR and quantitative RT PCR analyses.

Facility Manager: Karla Esbona Trip Lab Contact:

Phone: 608-265-9168 Hours: 6:30am-5pm M-F Email: [email protected]

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TSB - Experimental Animal Pathology Lab (EAPL)

The mission of the Experimental Animal Pathology Laboratory (EAPL) is to provide a comprehensive array of histopathology services and expertise that enable UWCCC members to fully exploit the power of animal models of human cancer to reveal mechanisms that contribute to cancer development, progression, and responsiveness to therapies. Contact Us at [email protected]

Facility Manager: Joe Hardin EAPL Lab Contact:

Phone: 608-262-1836 Hours: 8:00am-4:30pm M-F Email: [email protected]

Translational Science Biocore: TRIP Lab (TRIP)

A cellular image

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  • Tissue Microarray comes to TRIP…

Tissue Microarray comes to TRIP Lab

The TRIP Lab is offering tissue microarray (TMA) creation services with its brand new TMA Grand Master arrayer. This automated instrument can easily and quickly create TMA blocks with great precision and also extract paraffin embedded tissue samples to standard 0.2 ml PCR tubes to be used later in various molecular pathology applications. TMAs are a valuable, high-throughput method for diagnostic and research purposes. By being able to place hundreds of different samples into one paraffin block, TMAs bring major economies in time, quality and costs of tissue preparation, slide preparation and staining. For more information contact TRIP at [email protected] .

Categorized under:

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Pathology and Imaging

The Pathology and Imaging Core offers a number of services for investigators with funded NEI R01s and those planning on submitting an R01 to NEI. Among the services provided by the Pathology and Imaging Core are advanced histology through the Translational Research Initiative in Pathology TRIP) lab. Assistance with light and electron microscopy, confocal microscopy, live cell imaging, image analysis, and quantitative morphometry of tissue sections and cultured cells is also available (see list of services below).

The TRIP Lab provides routine histology, including paraffin and frozen processing, embedding, and sectioning. To submit a service request, please set up an account with iLab . They also do routine and some special histological staining. Please inquire at [email protected] for the stains. The histopathology services of the UW TRIP Lab are available at no charge to qualified NEI R01 investigators and investigators gathering preliminary data for their NEI R01 grant submissions.

Immunocytochemical and histochemical evaluations

Vision Core technical staff is also available to help embed samples in either paraffin, glycol methacrylate, Epon, OCT or gelatin. If there is something special that your research requires, please contact the Core Director – Dr. Donna Peters .

Our staff can help the investigator develop antigen retrieval procedures and post-fixation labeling studies. The client will provide any primary and secondary antibodies and/or linkers (e.g. streptavidin) necessary for visualization by light, fluorescence, or transmission electron microscopy. Expertise is available for a variety of labeling procedures, including live cell staining  to visualize and quantify the viability of cells in cultured anterior segments.

Morphometry

The Pathology and Imaging Core can assist with qualitative and quantitative morphometry utilizing well established histologic procedures to measure ganglion cell loss, apoptosis, loss of cell contacts, changes in cell morphology or the organization of the cellular matrix. For example, laser protection of retinal ganglion cells in experimental glaucoma can be determined by localizing the positions of surviving ganglion cells on serial sections through the laser spots and performing mathematical 2-D reconstructions and 3-D density plots.

The core also has  Zeiss Zen software  for morphometry. This software allows the investigator to generate composite images of large objects, measure length, distance, area, circumference and angles and do spot counts either manually or automatically.

Tissue culture cell depository

We have started a human eye tissue/cell depository. The goals of this service are to obtain human eye tissues and fluids (aqueous humor and vitreous) for PIs o use in their studies.  We can train investigators to isolate human eye cells or to provide “hard to isolate” human eye cells. To date, we have provided human trabecular meshwork cells (both normal and  immortalized ), primary human corneal keratocytes, and primary ciliary muscle cells for core participants.  We can also help   generate immortalized cell lines using a lentiviral vector expressing telomerase to expand the lifespan of human eye cells.

Subsidies for Service/Equipment Use for NEI R01 PIs and those who are submitting NEI R01 grants

Funding for fee based services or equipment on campus is available to NEI R01 investigators and PIs applying for NEI R01 grants.  Funding for fee based services or equipment use will be determined each year and will be based on the number of qualified users and the level of use. Once the allocated amount is exceeded, the PI will have to provide their own funding. PIs requesting these funds should contact the Vision Core Director to determine the level of funding that may be available. Note, these subsidies are to be used for data needed for  the PIs funded R01 project.

Subsidized Services Are :

  • Optical Imaging Core  (WIMR)
  • Electron Microscopy (WIMR)
  • TRIP lab services involving IHC, Multispectral Imaging and Slide Scanning technology, and Cell line Authentication (STR) (WIMR)
  • Tissue Availability from the Biocore  Biobank (WIMR)
  • X-Clarity tissue cleaning system (WIMR) – $100/run
  • Andor spinning disc confocal (MSC) – $30/hr

For questions regarding Pathology and Imaging services, please contact:

Pathology and Imaging Core Director

Donna M. Peters, PhD 1794 West Wedge, WIMR 1111 Highland Ave. Madison, WI 53706 Phone: 608-262-4626 Email:  [email protected]

Pathology and Imaging Core Assistant Director

Mrinalini Hoon, PhD 9457 WIMR II 1111 Highland Ave Phone: 608-263-6648 Email: [email protected]

Hongyu Noel, Core Instrument Manager 561A Bardeen 1300 University Ave. Madison, WI 53706 Phone: 608-262-8055 Email: [email protected]

Wisconsin Alumni Research Foundation

  • News & Media

Microscopy at UW-Madison. We use. We know. We innovate.

The university of wisconsin-madison and morgridge institute for research know microscopy. discover our innovative microscopy solutions., light/expansion microscopy.

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Overcome the diffraction limit inherent in light microscopy. Learn how new breakthroughs in expansion microscopy (ExM) enable investigators to examine small structures previously beyond the grasp of light microscopy.

Optimized Economical and Modulatable Isotropic Expansion Microscopy Aussie Suzuki, Mark Burkard, Roshan Norman, Emma Recchia This optimized set of protocols for better expansion of expansion microscopy (ExM) samples allows for 4 to 10-fold expansion of tissue cells and human organoid without expensive equipment or materials.

Light/Fluorescence Microscopy

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Fluorescent microscopes require frequent calibration, testing, and maintenance. Our researchers have developed novel tools that significantly simplify this process to ensure maximum performance from your instruments.

Microtubule Labeled Slides Used for Calibration of Various Fluorescence Microscopes Aussie Suzuki The microtubule slide can be used to calibrate any optical microscope, including light, confocal and super-resolution instruments.

Cryo-Electron Microscopy (cryo-EM)

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Sample preparation traditionally has been one of the most difficult and limiting aspects of cryo-electron microscopy (cryo-EM). We have developed new preparation methods that simplify this process while retaining sample integrity.

Biomolecular Vapor Deposition (BVD) and Grid Holder/Mounting System for In Vacuo Preparation of Cryo-Electron Microscopy Samples Joshua Coon, Michael Westphall This new cryo-electron microscopy (cryo-EM) sample preparation method combines biomolecular vapor deposition (BVD) with an improved TEM grid handling system.

Freezing and Jacketing Gas-Phase Biomolecules with Amorphous Ice for Electron Microscopy Joshua Coon, Michael Westphall The new cryo-electron microscopy (cryo-EM) sample preparation method, called biomolecular vapor deposition (BVD), is a gas-phase sample preparation system that uses mass spectrometry to mitigate many of the shortfalls associated with cryogenically fixing biological samples in amorphous ice.

Gas Phase Sample Preparation For Cryo-Electron Microscopy Joshua Coon, Michael Westphall This new cryo-electron microscopy (cryo-EM) sample preparation method addresses the limitations of current preparation methods and uses mass spectrometry to purify proteins and protein complexes.

Single-Molecule Microscopy

uw trip lab

Expand the use of single molecule microscopy beyond fluorescent molecules. These technologies enable microscopy to be performed on non-fluorescent single molecules, allowing study on a broader range of molecules.

All-Glass Optical Microresonator for Single Molecule Spectroscopy and More Randall Goldsmith, Kassandra Knapper, Kevin Heylman The improved optical microresonator platform shows superior biocompatibility and performance.

Microcavity Method for Single Molecule Spectroscopy Randall Goldsmith, Kevin Heylman The new microcavity-based method enables detection, identification and real-time analysis of single molecules and particles.

Applied Microscopy

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T cell activation plays a critical role in immunotherapy. Learn how new tools in the sorting and classification of T cells enable medical professionals to better characterize T cells and improve therapeutic efficacy.

Algorithms to Classify T Cell Activation by Autofluorescence Imaging Melissa Skala, Anthony Gitter, Zijie Wang, Alexandra Walsh The method and algorithms to non-invasively detect T cell activation by imaging NAD(P)H can be applied to NAD(P)H images taken with commercial imaging flow cytometers/sorters and fluorescence microscopes.

Method and Device to Screen and Sort Cancer Immunotherapy Cells Melissa Skala, Alexandra Walsh This non-invasive method uses the autofluorescence signals of NAD(P)H and FAD to determine T cell activation state without the use of contrast agents or requiring tissue/cell fixation.

Microscopy cores at UW-Madison

Cryo-Electron Microscopy Research Center (CEMRC) The CEMRC provides instrumentation, technical assistance, training and access to cryo-EM for the UW-Madison research community. The CEMRC manages and operates four cryo-microscopes for data collection by single particle, tomography and micro-ED. The microscopes are overseen by experienced staff who offer consultation and training in negative-stain and vitrified sample preparation, single particle analysis, tomography, data processing and additional computational support.

Translational Research Initiatives in Pathology (TRIP) Lab TRIP provides high quality services in cellular, molecular, quantitative and computational pathology to the UW campus research community and beyond. TRIP offers histological, histochemical, molecular and imaging analyses services such as tissue microarray (TMA) creation, imaging of slides in RGB and multispectral formats, IHC/ISH and cell line authentication on a fee for service basis.

Newcomb Imaging Center (NIC) NIC is a space for research and education in the department of botany that offers expertise, instruction and access to instrumentation in modern microscopy to the community of scientists at UW-Madison.

Optical Imaging Core The UW-Madison Optical Imaging Core offers investigators and biotech partners training and accessibility to advanced imaging technology.

UW Vision Research Core The UW-Madison Vision Research Core is funded by the National Eye Institute (NEI) and provides vision researchers three distinct service areas: quantitative molecular biology, pathology & imaging and animal models & eye organ culture.

Nanoscale Imaging and Analysis Center (NIAC) NIAC provides state-of-the-art instrumentation, support facilities and expert technical assistance for microscopy and microanalysis to support research and education in materials.

Scanning Election Microscope (SEM) Lab Scanning Electron Microscopy is an excellent method for providing high spatial resolution images on a wide range of samples. SEM provides high depth of focus images of 3-dimensional structures as well as compositional contrast images.

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Video Modal

Exploring relationship among sTRess, Isolation, and Physical activity (TRIP) in older adults living with HIV

Years: 2011-2013.

As life expectancy for persons living with HIV (PWH)  increased, morbidity and mortality from non-AIDS defining complications such as cardiovascular disease, cancer and neurological health conditions has also risen. These chronic co-morbidities, as well as HIV disease progression, might be linked to increased stress and isolation in PWH. There are related behaviors (physical activity, social activities, sleep hygiene) that can be modified through educational, societal and biomedical interventions, to decrease this stress and potentially slow the development of non-AIDS defining complications. The specific aims of this project were to:

  • Describe and compare levels of stress, isolation, and physical activity between older HIV+ adults (>51 years of age) and younger HIV+ adults (18-50 years); and
  • Describe and compare differences in levels of stress, isolation, and physical activity between men and women living with HIV/AIDS.

Research Design

TRIP was a descriptive longitudinal study with four age- and gender-stratified groups: 100 adult PLWHA in Northeast Ohio; 50 adults 18-50 years of age; 50 adults > 51 years of age.

All subjects completed baseline assessments of stress (psychometric scales, heart rate variability, and serum biomarkers) and isolation, as well as wrist actigraphy; 24-hour nutrition assessments; and sleep, medication adherence, and physical activity diaries. Subjects also completed an additional physiological assessment, psychometric scales, and consented to chart abstraction to assess covariates. Follow up assessments were made approximately 1 year after the baseline assessment and all data have been entered into the HIV Biorepository.

Research Team

Principal Investigator

  • Allison R. Webel , RN, PhD, FAAN, Frances Payne Bolton School of Nursing, Case Western Reserve University

Co-Investigators

  • Jan E. Hanson,  MPH, University Hospitals Case Medical Center, Comprehensive Cancer Center
  • Chris T. Longenecker,  MD, University Hospitals Case Medical Center; Harrington Heart and Vascular Institute
  • Robert A. Salata , MD, University Hospitals Case Medical Center; Case Western Reserve University School of Medicine

Research Publications

  • Webel, A. R ., Perazzo, J., Decker, M., Horvat-Davey, C., Sattar, A. and Voss, J. (2016),  Physical activity is Associated with Reduced Fatigue in Adults Living with HIV/AIDS.   Journal of Advanced Nursing . 72(12),3104-3112
  • Perazzo, J., Webel, A .  Alcohol Use and HIV Self-management.  (2016)  Journal of the Association of Nurses in AIDS Care . 28(2), 295-299.
  • Webel, A.R. , Sattar, A, Schriener, N., Kinley, B., Moore, SM. & Salata, RA. (2016)  The Impact of Mental Wellness on HIV Self-Management.     Journal of the Association of Nurses in AIDS Care.  27 (4) 468-75.
  • Webel, A.R. , Sattar, A. Schreiner, N. & Phillips, J.C. (2016).  Social Resources, Health Promotion Behavior, and Quality of Life in Adults Living with HIV Disease.  Applied Nursing Research 30, 204-209. NIHMS 714054
  • Webel, A.R. , Barkley, J.E., Longenecker, C.T., Mittlelsteadt, A., Gripshover, B., Salata, R.A. (2015).  A Cross-Sectional Description of Age and Gender Differences in Exercise Patterns in Adults Living With HIV .   Journal of the Association of Nurses in AIDS Care . 26(2) 176-186 PMCID PMC4284137
  • Webel, A.R.  Longenecker, C.T., Gripshover, B., Hanson, J.E., Schmotzer, B.J. & Salata, R.A. (2013).  Age, Stress, and Isolation in Older Adults Living with HIV.   AIDS Care . 1-9

Research Presentations

  • NINR 30th Anniversary Poster Presentation
  • Webel AR. , Sattar A, Longenecker, CT, Schreiner N, Josephson, R. (2015).  The Relationship Between Lifestyle Factors and Cardiovascular Disease Risk in HIV & Adults.  AHA Epidemiology/Lifestyle Scientific Sessions. March 5. Baltimore, MD. Poster Presentation (P232)
  • Webel, AR. , Longencker, CT., Hanson, JE. & Salata, R.  Exploring differences in sTRess, Isolation, Physical activity and sleep (TRIP) in older and younger adults living with HIV (PLWH) . Council on the Advancement of Nursing Science, Washington, DC. Poster Presentation

Media Publications

  • Stress and Isolation Take Toll on Those Under 50 With HIV: Older People Fare Better (TheBodyPro.com)
  • Studies: Younger HIV patients more isolated, stressed than older patients; life expectancy improving (Cleveland.com)
  • Exercise advised for HIV-positive people (International Federation of Gynecologists and Obstetrics/FIGO.org)
  • Regular exercise at home key to reducing chronic disease risk in people with HIV (The Nation’s Health, a publication of the American Public Health Association)
  • Home-exercise plan for HIV patients (News-Medical.net)
  • The HIV Mental Health Generation Gap (POZ.com)
  • Exercise is Linked to Lower Fatigue (POZ.com)

This study was supported by funds from the National Institutes of Health (5 KL2 TR000440, 1UL1 RR024989, & P30 NR010676) in the United States.

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PITCH YOUR IDEA and win a trip to Berlin! – FALLING WALLS LAB on May 16th

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The UW Office of Global Affairs, in collaboration with the German Center for Innovation & Science, the German Research Foundation, and UW CoMotion, would like you to know about the  Falling Walls Lab , a great opportunity for students to share their ideas and solutions for the challenges of our time.

The  Falling Walls Lab i s coming to Seattle on May 16th!

Which wall will your research break?  Pitch your innovation in just three minutes, showcasing an idea that could positively impact science and society.

This pitch-your-idea event is open to all students. All participants will pitch their idea (3 minutes) on Thursday, May 16 at CoMotion.  And  the winning individual/team will receive a fully paid trip to Berlin to meet with industry leaders and innovators.

The deadline for the submission is  Wednesday, May 1 . For more details and application instructions go to  https://falling-walls.com/lab/apply/seattle/  and see the attachments to this message.

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IDF fires artillery shells into Gaza as fighting between Israeli troops and Islamist Hamas militants continues on Oct. 12, 2023.

Middle East crisis — explained

The conflict between Israel and Palestinians — and other groups in the Middle East — goes back decades. These stories provide context for current developments and the history that led up to them.

Israel cancels high-level talks in Washington after cease-fire vote clears the U.N.

Jaclyn Diaz

Michele Kelemen 2010

Michele Kelemen

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The United Nations Security Council meets on the situation in the Middle East, including the war in Gaza, at U.N. headquarters in New York on Monday. Angela Weiss/AFP via Getty Images hide caption

The United Nations Security Council meets on the situation in the Middle East, including the war in Gaza, at U.N. headquarters in New York on Monday.

JERUSALEM — The United Nations Security Council has voted 14-0 in favor of a resolution demanding a cease-fire in Gaza for the rest of Ramadan. The United States abstained from the vote, clearing the way for the measure to pass.

The U.S. decision to abstain drew a swift response from Israeli Prime Minister Benjamin Netanyahu, who cancelled a visit by an Israeli delegation that had been set to travel to Washington, D.C., for talks on Israel's planned military operation in Rafah, in southern Gaza.

"Prime Minister Netanyahu made it clear last night that if the US withdraws from its principled position, he will not send the Israeli delegation to the US. In light of the change in the American position, Prime Minister Netanyahu decided that the delegation would not go," the prime minister's office said in a statement.

State Department spokesman Matthew Miller said Netanyahu's statement was "a bit surprising and unfortunate."

The U.S. abstention was seen as a sign of a growing rift between the two close allies. Washington is urging Israel not to launch an offensive in Rafah — where more than a million Palestinians are sheltering. Israel says it has to go in to destroy remaining Hamas battalions there.

The high-level delegation, led by Strategic Affairs Minister Ron Dermer and National Security Advisor Tzachi Hanegbi, was due to meet with Biden administration officials to hear U.S. concerns over the Rafah operation and discuss an alternative strategy.

Despite the cancellation, a planned visit by the Israeli defense minister, Yoav Gallant, continued. Gallant was in Washington on Monday to meet with Secretary of State Antony Blinken, Secretary of Defense Lloyd Austin and National Security Adivser Jake Sullivan.

What this resolution says

The cease-fire resolution calls for the immediate, unconditional release of all hostages taken captive by Hamas during the Oct. 7 attack on Israel that killed 1,200 people. Israel's military offensive in Gaza in response to the attack has killed more than 32,000 Palestinians, according to the Gaza Ministry of Health.

Ramadan is set to end in just over two weeks, on April 9, so if any cease-fire does manifest from the vote it may only be short-lived.

Monday's vote followed several failed attempts by the Security Council at brokering a cease-fire resolution — including one as recently as three days ago.

The U.S. had supported calls for a cease-fire only if they were directly connected to the release of some 130 hostages still in captivity under a deal being negotiated by diplomats from four nations.

"This resolution further explicitly recognizes the painstaking, non-stop negotiations being conducted by the Governments of Egypt, Israel, Qatar, and the United States to achieve such a release in the context of a ceasefire, which would also create space to surge more lifesaving humanitarian assistance for Palestinian civilians, and to build something more enduring," said Secretary of State Antony Blinken in a statement.

But because the final text of the resolution did not include "key language we view as essential, notably a condemnation of Hamas, we could not support it," Blinken added.

U.S Ambassador Linda Thomas-Greenfield is now urging Security Council members to put pressure on Hamas to accept a deal being negotiated in Doha.

There's already 'catastrophic' hunger in Gaza. Who decides when to call it a 'famine?'

Goats and Soda

There's already 'catastrophic' hunger in gaza. who decides when to call it a 'famine'.

"We're getting closer to a deal for an immediate cease-fire with the release of all hostages, but we're not there yet," said Thomas-Greenfield after the vote.

National Security Council spokesperson John Kirby was adamant that the vote did not reflect a change in U.S. policy.

"We have been clear and we've been consistent in our support for a cease-fire as part of a hostage deal," Kirby said on a call after the vote with reporters. He reiterated that because the final text did not include a condemnation of Hamas for the Oct. 7 attacks, the U.S. abstained rather than voting in favor of the resolution.

"It seems like the prime minister's office is choosing to create a perception of daylight here when they don't need to do that," Kirby said.

He added, "Of course we still have Israel's back."

Representatives for Hamas and Israeli are still in Qatar for indirect negotiations over a cease-fire, but remain far apart on several details.

Blinken urges Netanyahu not to attack Rafah as cease-fire resolution fails at the U.N.

Blinken urges Netanyahu not to attack Rafah as cease-fire resolution fails at the U.N.

Hamas said it welcomed the call from the Security Council for an immediate cease-fire, but called on the international body to pressure Israel to adhere to the cease-fire and stop the war.

U.N. Secretary General Antonio Guterres said, "This resolution must be implemented. Failure would be unforgivable."

Jaclyn Diaz reported from Jerusalem. Michele Kelemen reported from Washington, D.C.

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Marine Biology

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Research Vessels

There are two primary research vessels that UW undergraduates in marine biology may spend time on. In introductory classes, you may get a chance to go on a short overnight trip in the Puget Sound. If you major in a marine science field or spend a quarter at Friday Harbor Labs , your time may be focused on collecting data for your own research.

R/V Thomas G. Thompson

A 274 ft long vessel owned by the Office of Naval Research and operated by the UW School of Oceanography. Undergraduates may have the opportunity to tour the Thompson or go on day field trips in the local area. Oceanography majors collect data for their senior research projects through a senior research cruise, which frequently happens further afield in the Pacific Ocean. More information about the R/V Thompson .

In the News

  • “Oceanography team leads study of unexpected seafloor seep” (UW News, April 10, 2023)
  • “Annual research trip off Oregon coast gives students once in a lifetime experience at sea” (UW Environment, August 22, 2022)
  • “UW’s large research vessel, R/V Thomas G. Thompson, gets back to work” (UW Today, February 1, 2018)

R/V Rachel Carson

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A 72 ft long vessel owned by UW School of Oceanography and part of the UNOLS fleet. A successor to the R/V Barnes, R/V Rachel Carson is intended for research in Western Washington and British Columbia. More information about the R/V Rachel Carson .

  • “R/V Rachel Carson Stars in a Brand Story for Sea-Bird Scientific” (UW Oceanography, January, 2023)
  • “New UW vessel, RV Rachel Carson, will explore regional waters” (UW Today, May 10, 2018)

R/V Kittiwake

Kittiwake docked at FHL

A 42 ft long vessel stationed at Friday Harbor Labs on San Juan Island. More information about the R/V Kittiwake .

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Travel portal

Use the Travel portal to submit pre-trip authorization requests, to book a flight, to pay conference registration, and to submit a travel reimbursement request.

All postdoctoral scholars, graduate students, and staff must obtain approval prior to incurring any UW-covered travel expense. Prior approval is not required for faculty as this has been delegated to them by the Chair. 

Travel reimbursements must be submitted within 90 days of return from your trip. 

Complete travel policy for the Department of Chemistry:  Department Travel Policy  

Prior Approval

To obtain prior approval, postdocs, graduate students, and staff must complete the  Travel Request Form  (UW NetID required).

Please be sure your entry is complete. Your cost estimate should include all expenses for the entire trip – transportation, lodging, per diem, and registration fees. The request will be routed electronically via UW Connect to a travel specialist in the Chemistry Purchasing & Accounting Office who will review the form, request additional information or clarification if needed. If you already have supervisor approval, please attach it to your REQ. If supervisor approval is not provided, we will route for approval.

Once approval is secured, the travel specialist will authorize charges to the Department Corporate Travel Account as needed. Upon completion and return from travel, the travel specialist  will work with the traveler to prepare a travel Expense Report for reimbursements.

Faculty Travel

Prior approval is not required for faculty travel. For reimbursement, faculty should use the travel portal at top of page.

Travel reimbursements are handled by the travel specialist in the Chemistry Purchasing & Accounting Office (109P Bagley Hall). Please provide the travel specialist through the portal  the complete information, receipts, and supporting documentation shortly after travel has been completed.

International Travel

All graduate and undergraduate students are required to register international travel itineraries with the Office of Global Affairs. The  UW Office of Global Travel  facilitates safe and successful travel for UW faculty, staff, and students. Students are also required to obtain UW Student Abroad Insurance. This provides major medical insurance benefits while overseas and evacuation services for medical, security, and natural disaster emergencies, which is not provided by regular GAIP insurance.

Please see the  UW Office of Global Affairs website  for more information.

Chemistry Corporate Travel Account

The Department has a corporate travel account (CTA) for airline, other transportation and specified lodging. If you wish to have the Department pay for any travel expenses in advance of your trip with the CTA account, complete the  Request to Charge CTA form (PDF)  (fillable pdf). 

Send the completed request form to [email protected]  in the Chemistry Purchasing & Accounting Office. Please note the prior approval requirement outlined above. 

Travel Basics

  • See our  Travel FAQ document (PDF)  for answers to common questions related to UW travel.
  • Plan ahead. This ensures the desired flight times, best prices, and preferred accommodations.
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  • Business or first-class travel is allowable only under very special circumstances and with prior approval from the Dean of the College of Arts & Sciences. Contact Angie Mullen ( [email protected] , 206.685.2333) for more information.
  • International travel supported by Federal funds must use a U.S.-based air carrier (e.g., Alaska or American instead of British Airways; United instead of Air Canada; Delta instead of KLM). Contact the travel specialist, Patrick Arroyo (109P Bagley Hall,  [email protected] , 206.897.1894) or Angie Mullen ( [email protected] , 206.685.2333) if you have questions.
  • As soon as possible following your return, submit your reimbursement request to the travel specialist, Patrick Arroyo  (109P Bagley Hall,  [email protected] , 206.897.1894)  . 

UW Travel Cards

Faculty or staff who take at least two business trips each year may want to consider obtaining a UW Travel Card. This is an individual liability card, i.e., the card holder is responsible for paying the expenses incurred. As long as reimbursements are filed in a timely fashion, the Travel Card bill will typically not be due before you receive your reimbursement. Unfortunately, the UW Travel Card is not available to student employees. Please see the  UW Travel Office website  for more information about the UW Travel Card. 

Please direct any questions to the travel specialist, Patrick Arroyo  (109P Bagley Hall, [email protected] , 206.897.1894)  or Angie Mullen ( steuerma @uw.edu , 206.685.2333) in the Chemistry Purchasing & Accounting Office.

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Kwik Trip will discontinue bagged milk in May

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Kwik Trip and bagged milk.

It was a partnership going back more than 40 years, however the convenience store chain, founded in Wisconsin , announced Wednesday that Nature's Touch bagged milk will be discontinued at all stores beginning in May.

Kwik Trip said in a news release that "guest preferences and purchasing patterns have indicated a substantial declining demand for this product compared to milk sold in jugs."

John McHugh, vice president of external relations, said Kwik Trip has begun to contact groups who had special promotional coupons for bagged milk and have offered a new solution with other milk offerings.

"This is an end to a product that was associated with the Kwik Trip brand for many years, but after evaluating the need and cost for new equipment to replace our antiquated bagged milk equipment, and the decreased customer demand, we made the tough decision to discontinue Nature’s Touch milk in the bag," McHugh said.

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As some newsroom roles go the way of the dinosaurs, brand new jobs are being born. This interview is part of an occasional series of Q&As with people who are the first to hold their title in their newsroom. Read through the rest here .

black and white headshot of phoebe connelly, senior editor for AI strategy and innovation at the Washington Post

At the Post, Connelly’s job, first and foremost, is to experiment. She’ll be overseeing the development of tools and processes that bring generative AI into the newsroom, at all levels, while maintaining editorial standards.

It’s no small task. Several outlets have already set prime examples for what not to do when it comes to AI adoption. Shoddy copy, PR crises, and factual inaccuracies have plagued early adopters of ChatGPT, such as Sports Illustrated , CNET , and Gizmodo .

But after years leading the Post’s Next Generation initiative to bring younger readers to the publication — another first-of-its-kind role — Connelly says she’s up for the challenge. I chatted with her over email to learn more about what led her to this unique role, and what she hopes to do with it. Our conversation has been lightly edited for length and clarity.

Andrew Deck : How “first” is this position? Is it the first time that someone has held the title of editor for AI strategy and innovation in your newsroom? Have you seen other newsrooms create similar positions?

Phoebe Connelly : I’m the first person with this title at The Washington Post, and after me the title is going to a LLM. (Kidding, I hope.)

As with most new roles, there is a need for my role because of the many people who are already working with AI and want to see us move faster. Meghan Hoyer , head of data journalism in the newsroom, has an incredible track record and I’m very fortunate to now get to be in weekly meetings with Sam Han , who is the director of AI on our engineering team.

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Deck : What is your job?

Connelly : Working across our newsroom and product teams to launch ambitious, delightful AI news experiences for our users. Getting our newsroom access to AI-powered tools that let us do our jobs faster. Getting to convene complex conversations about how we employ this new tool in ways that meet our rigorous standards.

I have a dual reporting structure to [executive editor] Sally Buzbee and [chief technology officer] Vineet Khosla — our hope is this cross-functional structure will ensure we are pulling from the whole company to build with generative AI.

Of course, first on my list was how we can better serve our readers.

Deck : What did working with AI look like in The Washington Post newsroom before this role was created? How do you expect that to change?

Connelly : Machine learning and artificial intelligence have been employed by the Post for years, and we are fortunate enough to have many skilled technologists on staff.

Last year, the Next Generation team started some experiments that leveraged generative AI. We held a company wide hackathon and our product manager, Tony Guzman started prototyping news delivery surfaces that incorporated generative AI.

The announcement of a new role is a moment that gets everyone thinking about what is possible. Now my colleagues know to come to me with ideas they’d like to see us pursue. Newsrooms are deeply collaborative places, used to forming ad hoc teams around a breaking story or pairing up to maximize reporting resources. Tackling a new technology in that type of collaborative environment is thrilling.

Deck : What previous experiences — personal, professional, educational — led you to this job?

Connelly : In my first role at the Post — as a producer on the video team — I was asked to attend a meeting about our video player that turned into multiple meetings, which turned into a proposal to build a new video CMS. That CMS is now part of Arc XP .

Getting a crash course in product development was formative. My partner in building video products was [director of engineering] Vidya Viswanathan . She was so generous with her time, willing to talk through any problem and eager to figure out how to work with the particular rhythm of a newsroom. I learned so much working with her. We sit right next to each other again — it’s like coming home.

Building the Next Gen team really forced me to think through how an organization can tackle new problems while maintaining its core values. And I got to work with simply the most talented slice of people across the Post. We as an industry should set up more short-term teams. It was incredible to work fast and hard at a problem for two years and see what change we could accomplish.

My dad just retired from the Chicago Transit Authority . In his farewell speech he said, “The glass is always half full for me.” I am definitely my father’s daughter. I’m always looking for the upside, the opportunity. And yes, if we take a business trip together I will make you take public transit.

Deck : There is a lot of anxiety in journalism at the moment around job displacement due to AI adoption. How do you plan on addressing anxieties in your own newsroom?

Connelly : We all need to encourage experimentation with generative AI. Once it stops being an idea and becomes a tool, then we can move on to the fun part which is figuring out what uses we can put it to.

I’m not afraid of AI as a journalist. We are so good at leveraging new tools to report and deliver the news. Generative AI is just the latest. Journalists introduce new facts into the conversation, and we do this through multiple-sourced, transparent reporting. This skill set and our core values are even more valuable in an AI-mediated landscape.

Deck : How do you think about newsroom talent as it relates to AI? In your role, will you be training up journalists at The Washington Post on how to best use AI tools, or also bringing prompt engineers and other AI specialists into the newsroom to use these tools?

Connelly : We just completed our first round of prompt training in the newsroom! We were led by the excellent David Caswell — his piece for the Reuters Institute is great reading if you are trying to figure out where to start.

Deck : What do you see as some of the challenges and opportunities for being the first AI strategist — or the first anything — at a news organization?

Connelly : This is my second “first-of-a-kind” position. The Next Gen team was a totally new type of team for the Post. I found it helpful to remind myself that we had two jobs, where a lot of teams only had one: We had to define what our mission was, and we had to execute on it. Most jobs, you are handed the plan, the mission, when you start. I find thinking about it as a two part job makes the wild range of tasks you might face in a week make more sense.

First-of-their-kind jobs are as much about culture change as they are about the task. It’s not enough that I get us thoughtfully using generative AI — I need to leave the newsroom feeling good about how we got there.

Photo of Phoebe Connelly by Elliot O’Donovan, courtesy of The Washington Post. Hedge maze illustration created with Midjourney.

Cite this article Hide citations

Deck, Andrew. "The Washington Post’s first AI strategy editor talks LLMs in the newsroom." Nieman Journalism Lab . Nieman Foundation for Journalism at Harvard, 28 Mar. 2024. Web. 28 Mar. 2024.

Deck, A. (2024, Mar. 28). The Washington Post’s first AI strategy editor talks LLMs in the newsroom. Nieman Journalism Lab . Retrieved March 28, 2024, from https://www.niemanlab.org/2024/03/the-washington-posts-first-ai-strategy-editor-talks-llms-in-the-newsroom/

Deck, Andrew. "The Washington Post’s first AI strategy editor talks LLMs in the newsroom." Nieman Journalism Lab . Last modified March 28, 2024. Accessed March 28, 2024. https://www.niemanlab.org/2024/03/the-washington-posts-first-ai-strategy-editor-talks-llms-in-the-newsroom/.

{{cite web     | url = https://www.niemanlab.org/2024/03/the-washington-posts-first-ai-strategy-editor-talks-llms-in-the-newsroom/     | title = The Washington Post’s first AI strategy editor talks LLMs in the newsroom     | last = Deck     | first = Andrew     | work = [[Nieman Journalism Lab]]     | date = 28 March 2024     | accessdate = 28 March 2024     | ref = {{harvid|Deck|2024}} }}

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It’s a project of the Nieman Foundation for Journalism at Harvard University .

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Forget rest stops. Plan your road trip around playgrounds.

When toddler meltdowns strike, google maps is your friend.

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Welcome to The Upgrade, By The Way’s series on travel hacks and hot takes. See how to submit here.

I don’t know where my 3-year-old picked up the phrase. Maybe it’s innate to all kids who’ve been stuck in the car for more than 10 minutes: “Are we there yet?”

Unfortunately for all of us, the answer is almost always “No.” We won’t be there for many, many hours. That’s when a playground pit stop can save the day.

Over the past few years, my husband and I have driven round-trip between Washington, D.C., and Florida nine times to visit family, see friends, take vacation, work remotely and thaw out in the winter. Since we break the trips into manageable chunks for young kids and elderly dogs, that equals more than a month on the road.

We started building playground stops into the schedule once our daughter was almost a year and a half old and needed more mental and physical stimulation than exploring the front seat during rest stops. It’s become an essential part of long drives now, giving us — and most importantly, our energetic passenger — a light in the middle of the road trip tunnel. We can answer her “Are we there yet?” question by saying we’re 30 minutes from a playground, even if the final stop is a day and a half away.

In the early days, we just wanted convenient spots for diaper changes and toddling around, with maybe a baby swing as a bonus. Now that we have a new baby and a preschooler, we’re thrilled to find tall slides, big-kid swings, bridges and bathrooms — or at least space to set up a tiny portable toilet.

There have been deserted, tucked-away neighborhood parks where we ran into fellow travelers. And some have been bustling with locals, giving us a unique view into a city’s culture. The unicorn of stops would be near the highway with good takeout food nearby, a playground, a restroom and a dog park.

We’ve come to appreciate the train-themed Jefferson Park , perched on a hill in Richmond with a majestic view of the city and proximity to a great local coffee shop . James F. Holland Memorial Park in Palm Coast, Fla., clicked every box we had and then some. Our daughter’s playground adventures have included testing the little-kid swings in Savannah’s Forsyth Park, playing an oversized xylophone in Palm Coast, braving a mini rock-climbing wall in Richmond, rocking in a giant ladybug near Brunswick, Ga., and taking her scooter for a spin in Port St. John, Fla.

On a shorter trip — or in a playground-less wasteland — we’ve found that a rest stop lawn might substitute. If there are dandelions to be picked and scattered, it might even be preferred. On a recent gas-food-bathroom break between New York City and D.C., an empty Starbucks patio served a greater purpose. My husband and 3-year-old used it as an obstacle course so she could run around while I fed the baby in the car.

There are plenty of resources to help pick a playground: Tripadvisor recommendations , Reddit threads , parenting blogs and an app called Playground Buddy . When we set off in the mornings, I try to figure out how far we’ll get in three hours and scout a good spot there. But because children require more of a go-with-the-flow approach, I mostly operate in the moment.

So I fumble around Google Maps to find the nearest playground as the baby wakes up from a nap crying, and then the 3-year-old needs a bathroom break pronto. If I have time, I check reviews for reports of broken swings or dirty restrooms. Sometimes I manage to search for a Starbucks and gas station nearby so we can maximize the stop.

I’m sure there will be a day when we can power through the long drive as models of efficiency, stopping only for fuel and relief. But I’ll miss the playground detours and those little snapshots of my kids’ joy. We may not be “there” yet, but at least it’s somewhere fun.

More travel tips

Vacation planning: Start with a strategy to maximize days off by taking PTO around holidays. Experts recommend taking multiple short trips for peak happiness . Want to take an ambitious trip? Here are 12 destinations to try this year — without crowds.

Cheap flights: Follow our best advice for scoring low airfare , including setting flight price alerts and subscribing to deal newsletters. If you’re set on an expensive getaway, here’s a plan to save up without straining your credit limit.

Airport chaos: We’ve got advice for every scenario , from canceled flights to lost luggage . Stuck at the rental car counter? These tips can speed up the process. And following these 52 rules of flying should make the experience better for everyone.

Expert advice: Our By The Way Concierge solves readers’ dilemmas , including whether it’s okay to ditch a partner at security, or what happens if you get caught flying with weed . Submit your question here . Or you could look to the gurus: Lonely Planet and Rick Steves .

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Middle East Crisis U.S. Says Israel Has Agreed to Try to Reschedule Canceled Trip

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  • Mourning the dead in Rafah, in the southern Gaza Strip. Ahmad Hasaballah/Getty Images
  • A patient carried from an Israeli military helicopter in Tel Aviv. Carlos Garcia Rawlins/Reuters
  • Vendors in Gaza City amid destroyed buildings. Agence France-Presse — Getty Images
  • Aid airdropped into northern Gaza. Ohad Zwigenberg/Associated Press
  • An emergency center in al-Habbariyeh, Lebanon, destroyed in an overnight attack, according to the country’s official news agency. Mohammad Zaatari/Associated Press
  • Mementos and pictures of hostages kidnapped on Oct. 7, in Dizengoff Square in Tel Aviv. Hannah Mckay/Reuters
  • Palestinians searching through the rubble of a building that was destroyed overnight in Rafah. Mohammed Abed/Agence France-Presse — Getty Images

The U.S. says Israel has agreed to try to reschedule a canceled trip.

A White House spokeswoman said on Wednesday that the Israeli government had agreed to try to reschedule a visit by a group of officials whose trip to Washington to discuss a possible assault on a key southern city in Gaza was scrapped over the U.S. decision not to veto a U.N. resolution calling for an immediate cease-fire.

President Biden had asked Israel to send a delegation to Washington to discuss alternatives to a ground offensive in Rafah, the southern Gaza city where more than a million people have sought refuge. But Israel’s prime minister, Benjamin Netanyahu, called off the delegation’s trip at the last minute after being angered by the U.S. decision to abstain from a vote on the resolution at the U.N. Security Council on Monday.

“The prime minister’s office said that they want to reschedule this meeting so that we can talk about the Rafah operations,” the White House press secretary, Karine Jean-Pierre, told reporters on Wednesday. “We welcome that. And we’re going to work with their teams to make sure that happens.”

John F. Kirby, a White House spokesman, added on Thursday that the administration was working with the prime minister’s office and other Israeli officials to finalize a date for the rescheduled meeting. “We’re hoping that this meeting can be scheduled in person here in Washington as was the original plan,” he told reporters.

There was no immediate confirmation of a desire to reschedule from Mr. Netanyahu’s office, which, just hours before Ms. Jean-Pierre’s comments, had issued a statement denying reports that a meeting was back on. “Contrary to reports, the prime minister didn’t approve the departure of the delegation to Washington,” the statement said.

On three prior occasions, the United States had vetoed a cease-fire resolution. But by abstaining on Monday, it allowed the resolution, which was less strongly worded than previous ones and called for a cease-fire for the holy month of Ramadan, to pass.

Mr. Netanyahu denounced the abstention in a statement, calling it “a retreat from the consistent American position since the beginning of the war.” The Biden administration insisted on Monday that the abstention did not signify a change in the United States’ position.

Friction between the two allies has increased over the toll on civilians in Gaza after more than five months of fighting set off by the Hamas-led Oct. 7 attack, which, according to Israeli officials, killed about 1,200 people.

Health officials in Gaza say that more than 32,000 people have died during the Israeli military operation, and the fighting has created dire conditions on the ground, with humanitarian groups warning of a looming famine.

Asked about Mr. Netanyahu’s earlier denial of reports that the meeting would be back on, Ms. Jean-Pierre was adamant that his office had agreed to try to reschedule.

“When we have a date, certainly we will share that with you,” she said. “That is what we know from our side.”

The announcement came a few hours after Israel’s defense minister, Yoav Gallant, wrote on social media that he had completed a successful visit to the United States. The trip coincided with the U.N. vote and its fallout.

During his visit, Mr. Gallant met with several senior U.S. officials, including Jake Sullivan, the national security adviser, and Defense Secretary Lloyd J. Austin III, who made Rafah a central part of his agenda.

After the meeting, a senior Defense Department official said Mr. Austin had presented the broad outlines of the Biden administration’s alternative approach to a major combat operation in Rafah, including a focus on precision targeting intended to root out Hamas leadership.

The official, who spoke on a call with reporters on the condition of anonymity to discuss confidential talks, said that the Israelis were receptive and that there would be additional meetings in the future.

Ms. Jean-Pierre said the United States remained hopeful that it could help broker a temporary cease-fire and a release of hostages held by Hamas.

In an interview with Israel’s Channel 12 aired Wednesday night, Mr. Kirby acknowledged that the talks were stalling.

“We felt like the gaps were closing, and that we were getting closer to having a deal where we can get those hostages out,” he said. “Now it appears that we’re not moving forward, at least not in the way that we all had hoped, but that doesn’t mean we’re going to give up the effort.”

Johnatan Reiss and Katie Rogers contributed reporting.

— Zach Montague reporting from Washington

A 25-year-old was killed in Israel, while 7 were killed overnight in Lebanon.

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Hezbollah fired dozens of rockets into northern Israel on Wednesday, killing at least one person in a barrage that it said was retaliation for an Israeli strike that the authorities said killed seven medics overnight in southern Lebanon.

For months, Hezbollah and Israel have traded fire across the Israel-Lebanon border. The violence has displaced tens of thousands of people from their homes.

The Israeli military said on Wednesday that it carried out an overnight strike targeting a “significant terrorist operative” and others who were with him near the town of al-Habbariyeh in southern Lebanon. Lebanon’s health ministry said the strike hit an emergency medical center and killed seven paramedics and denounced what it called an “unacceptable” attack on a health center.

Hezbollah’s response was swift: It launched the volley of rockets into northern Israel in retaliation for the Habbariyeh strike and to show solidarity with Palestinians in Gaza, the group said. Magen David Adom, Israel’s nonprofit emergency medical service, said a direct hit on a building in the Israeli city of Kiryat Shmona had killed a 25-year-old.

Later on Wednesday, the Israeli military said its fighter jets had struck Hezbollah compounds in two areas of southern Lebanon, though it was not clear if the attacks resulted in any casualties.

Hezbollah is a key ally of Hamas, whose Oct. 7 attacks on Israel led to the war in Gaza. Since October, Hezbollah has been firing rockets into northern Israel on a near-daily basis. The Israeli military regularly responds with strikes against Hezbollah-linked targets inside Lebanon.

Both Hezbollah and Hamas are backed by Iran, and the clashes along Israel’s border with Lebanon have raised concerns that the war in Gaza could erupt into a wider regional conflict. Hezbollah’s attacks so far have been big enough to demonstrate the group’s solidarity with Hamas but measured enough to avoid provoking a war with Israel.

Kiryat Shmona, where the 25-year-old was killed on Wednesday, used to be home to about 24,000 people, but only about 1,500 inhabitants remain. Many residents, now scattered among 220 hotels across Israel, did not even wait for the government’s order on Oct. 20 to evacuate.

Rawan Sheikh Ahmad and Isabel Kershner contributed reporting.

— Cassandra Vinograd and Hwaida Saad

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Maps: Tracking the Attacks in Israel and Gaza

See where Israel has bulldozed vast areas of Gaza, as its invasion continues to advance south.

Two days after the U.N. call for a cease-fire, Israeli strikes on Gaza haven’t let up.

Israel’s air force on Wednesday continued to pound the Gaza Strip with strikes, and Hamas fighters kept up attacks against Israeli soldiers, a further indication that a United Nations Security Council resolution this week calling for a cease-fire had failed to persuade either side as attempts for an agreement appeared to falter .

Over the two days since the U.N. resolution passed on Monday, the Qassam Brigades, Hamas’s military wing, has said it is continuing to carry out attacks against Israeli soldiers. The Israeli military said on Wednesday that warplanes had hit dozens of targets over the previous day, including tunnels, military compounds and militants.

Israel has been outspoken in its condemnation of the Security Council resolution, which called for a cease-fire for the remaining weeks of Ramadan that would lead to a “lasting, sustainable” halt in the fighting and the unconditional release of all hostages held by militants in Gaza. The United States, which has vetoed previous attempts, abstained, allowing the resolution to pass.

Israel’s prime minister, Benjamin Netanyahu, met in Jerusalem on Wednesday with Senator Rick Scott, Republican of Florida, and continued to express defiance over the U.S. decision. He argued, according to a statement from his office, that it encouraged “Hamas to take a hard line and to believe that international pressure will prevent Israel from freeing the hostages and destroying Hamas.”

Israel and Hamas appear no closer to negotiating a stop in fighting, with significant gaps remaining between them.

On Wednesday, three Palestinian human rights groups said that there had been an intensification of Israeli bombardments on Rafah over the previous 72 hours, killing dozens. Hundreds of thousands of displaced Gazans are sheltering there. Some of the strikes described by the groups occurred after the Security Council’s resolution passed, while several others took place prior.

Gazan authorities reported on Wednesday that Civil Defense teams had pulled Palestinians out of the rubble after strikes in the Jabaliya neighborhood of northern Gaza, though the timing was unclear.

The Palestine Red Crescent Society said its teams had picked up the bodies of two people killed by artillery fire in the Nuseirat neighborhood.

The Israeli military did not immediately respond to a request for comment on the reports.

On Wednesday afternoon, Hamas said that it hit a soldier in the area surrounding Al-Shifa Hospital in Gaza City with sniper fire, after saying Tuesday that it had targeted two Israeli tanks in the Khan Younis area, and an armored personnel carrier and a soldier on the coastal north-south road.

Since early last week, Israeli forces have been raiding Al-Shifa in what the military has said is an effort to crack down on Hamas. Humanitarian organizations have expressed alarm over the situation at the medical facility, which, along with the surrounding area, had been sheltering thousands of people.

Over the last 48 hours, the Qassam Brigades has also published videos purporting to show militants firing on Israeli forces, but it was not clear when the videos were taken.

— Adam Rasgon Reporting from Jerusalem

A majority of Americans disapprove of Israel’s actions in Gaza, a new poll shows.

A majority of Americans disapprove of Israel’s military actions in Gaza, in a pronounced shift from November, according to a new poll released by Gallup on Wednesday.

In a survey conducted from March 1-20, 55 percent of U.S. adults said they disapproved of Israel’s military actions — a jump of 10 percentage points from four months earlier, Gallup found.

Americans’ approval of Israel’s conduct in the war dropped by an even starker margin, from 50 percent in November, a month after the war began, to 36 percent in March, while the proportion of Americans who said they had no opinion on the subject rose slightly to 9 percent from 4 percent.

The findings are the latest evidence of growing American discontent with Israel over the course of the five months in which it has killed more than 32,000 Palestinians in Gaza, including nearly 14,000 children, according to local health officials and the United Nations. Israeli officials say roughly 1,200 people were killed in Israel during the Hamas-led attack on Oct. 7.

The Gallup poll found that American approval of Israel’s military actions dropped across the political spectrum: While a majority of Republicans still said they approved, that figure dropped from 71 percent in November to 64 percent in March. Independents’ approval dropped to 29 percent from 47 percent, and Democrats’ approval dropped to just 18 percent from 36 percent.

An AP-NORC poll conducted in late January found that half of U.S. adults felt Israel’s military response in Gaza had “gone too far,” up from four in 10 in November. That poll also showed a rise in public disapproval across political parties, by some 15 percentage points for Republicans, 13 for independents and five for Democrats.

Another recent survey from the Pew Research Center — which, like Gallup and AP-NORC, is a well-regarded leader in the polling industry — found notable schisms in public opinion along generational and religious lines. Younger adults and Muslim Americans were significantly more likely than older adults and Jewish Americans to say that the way Israel was carrying out its response to Hamas’s Oct. 7 attack was unacceptable, according to the poll conducted from mid-to-late February.

It oversampled Muslim and Jewish Americans, weighted to reflect their respective proportion of the overall population, to more reliably and separately analyze their views.

— Anushka Patil

Security forces clash with pro-Palestinian protesters in Jordan, video shows.

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Security forces clashed with large crowds of pro-Palestinian protesters near Israel’s embassy in the Jordanian capital on Tuesday night, video recorded by The Associated Press and Reuters showed.

It was the third consecutive day that demonstrations in support of Palestinians under Israeli bombardment in Gaza have taken place near the embassy in Amman, Jordan’s capital.

The protesters carried Palestinian flags and marched toward the embassy, chanting “betrayal” in Arabic and calling on Jordan’s government to cancel its agreements with Israel.

Footage showed the security forces dispersing the crowds and arresting protesters.

Israel and Jordan maintain a crucial regional alliance, and the kingdom is the custodian of the Aqsa compound in Jerusalem, a key holy site that is often a source of disputes and conflict between Israelis and Palestinians.

But more than 2.3 million registered Palestinian refugees live in Jordan — a population slightly larger than that of the Gaza Strip. And Jordan’s leaders have been increasingly critical of Israel since the war in Gaza began. Amid a dire humanitarian crisis in the enclave, Jordan began airdropping aid in November. It has completed more than a dozen missions since.

— Cassandra Vinograd

Gazan authorities say 12 people drowned trying to retrieve airdropped aid.

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The authorities in Gaza said late Tuesday that a total of 12 people had drowned while trying to retrieve airdropped aid that had fallen into the Mediterranean, calling for an end to the airdrops — a last resort to get urgently needed food and other supplies into the enclave — and an increase in deliveries by land.

People waded into the water from a beach in northern Gaza on Monday afternoon to get the aid packages, according to Ahmed Abu Qamar, a Gaza-based researcher for EuroMed Rights, a human rights group, who said he had spoken to witnesses. He also said that around a dozen people had drowned, saying that at least one had become entangled in a parachute.

It was not possible to confirm the details independently and it was not clear which country was responsible for the airdrop in question.

Three of approximately 80 aid bundles dropped by the United States on Monday “were reported to have had parachute malfunctions and landed in the water,” a Pentagon spokeswoman, Sabrina Singh, said at a news conference on Tuesday.

The aid was intentionally dropped over water and intended to be carried to land by wind drift, to mitigate potential harm in the event that the parachutes failed to deploy, Ms. Singh said.

Karine Jean-Pierre, the White House press secretary, said on Wednesday that the Biden administration expressed “condolences to the families of those who died.” But she did not confirm whether the aid packages that had fallen into the sea were dropped by the United States.

The fatalities were not the first connected to aid drops. Earlier this month , the authorities in Gaza said that at least five Palestinians had been killed and several others wounded when airdropped aid packages fell on them in Gaza City. On Tuesday, the Gaza government media office said that six other people had died during what it characterized as stampedes as they tried to get aid that was airdropped in other locations.

The United Nations and other aid organizations say that trucks, rather than planes, are the cheapest, safest and most effective means of delivering aid to Gaza, a territory whose population of more than two million faces a hunger crisis that humanitarian organizations say borders on famine.

But several governments, including those of the United States, France, Jordan and Egypt, have in recent weeks used airdrops to supplement aid that arrives by land, while also calling on Israel to allow in more trucks.

Britain airdropped aid to Gaza for the first time on Monday, delivering over 10 tons of supplies along the northern coastline as part of a mission led by Jordan, the British defense ministry said in a statement .

Governments say that the drops are necessary because of a steep fall in the amount of aid entering Gaza since Oct. 7, when Hamas led a deadly attack on Israel. The number of aid trucks entering Gaza since then has fallen by around 75 percent, according to U.N. data. One charity, World Central Kitchen, delivered a bargeload of aid to Gaza earlier this month.

Governments and aid groups say Israel has slowed aid deliveries through stringent inspections of trucks. The authorities in Israel blame UNRWA, the United Nations aid agency that supports Palestinians, arguing that Israel can inspect and process aid trucks faster than humanitarian groups can distribute the aid inside the territory.

Abu Bakr Bashir , Adam Sella , Anushka Patil and Zach Montague contributed reporting.

— Matthew Mpoke Bigg

Who was Marwan Issa, the Hamas commander killed by Israel?

The Israeli military has confirmed that Marwan Issa, the deputy commander of Hamas’s military wing in Gaza and a presumed mastermind of the Oct. 7 assault on southern Israel, was killed in an Israeli airstrike this month.

A senior U.S. official, Jake Sullivan, had previously told reporters that Mr. Issa, one of the highest-ranking officials in Hamas, had been killed. But before a statement Tuesday , Israel’s military had said only that its warplanes had targeted Mr. Issa and another senior Hamas official in an underground compound in central Gaza.

With his death, Mr. Issa, who had been among Israel’s most wanted men, became the senior-most Hamas leader to be killed in Gaza since the start of the war. Israeli officials have characterized the strike as a breakthrough in their campaign to wipe out the Hamas leadership in Gaza.

But experts cautioned that his death — which Hamas has still not acknowledged — would not have a devastating effect on the armed group’s leadership structure. Israel has killed Hamas’s political and military leaders in the past, only to see them quickly replaced.

Here is a closer look at Mr. Issa and what his death means for Hamas and its leadership.

What was Mr. Issa’s role in Hamas?

Mr. Issa, who was 58 or 59 at the time of his death, had served since 2012 as a deputy to Mohammed Deif, the elusive leader of the Qassam Brigades, Hamas’s military wing. Mr. Issa assumed the role after the assassination of another top commander, Ahmed al-Jabari.

Mr. Issa served both on Hamas’s military council and in its Gaza political office, overseen by Yahya Sinwar, the group’s highest-ranking official in the enclave. Mr. Issa was described by Palestinian analysts and former Israeli security officials as an important strategist who played a key role as a liaison between Hamas’s military and political leaders.

Salah al-Din al-Awawdeh, a Palestinian analyst close to Hamas, described Mr. Issa’s position in the group as “part of the front rank of the military wing’s leadership.”

Maj. Gen. Tamir Hayman, the former Israeli military intelligence chief, said Mr. Issa was simultaneously Hamas’s “defense minister,” its deputy military commander and its “strategic mind.”

What does his death mean for the group?

Experts described Mr. Issa as an important associate of Mr. Deif and Mr. Sinwar’s, though they said his death did not represent a threat to the group’s survival.

“There’s always a replacement,” Mr. Awawdeh said. “I don’t think the assassination of any member of the military wing will have an effect on its activities.”

Michael Milshtein, a former Israeli military intelligence officer and an expert on Palestinian affairs, said Mr. Issa’s death was a significant blow to the Qassam Brigades, though he conceded it wasn’t “the end of the world” for Hamas.

“He had a lot of experience,” Mr. Milshtein said. “His death is a big loss for Hamas, but it isn’t a loss that will lead to its collapse and it won’t affect it for a long time. In a week or two, they’ll overcome it.”

Mr. Milshtein added that even though Mr. Issa’s opinion was valued at the highest levels of Hamas, the fact he did not directly command fighters meant that his death did not leave a gaping hole in Hamas’s operations.

How has he been described?

Mr. Issa was a lesser-known member of Hamas’s top brass, maintaining a low profile and rarely appearing in public.

Gerhard Conrad, a former German intelligence officer who met Mr. Issa more than a decade ago, described him as a “decisive and quiet” person lacking charisma. “He was not very eloquent, but he knew what to say, and he was straight to the point,” Mr. Conrad said in an interview.

Mr. Conrad said he met Mr. Issa, Mr. al-Jabari and Mahmoud al-Zahar, another senior Hamas official, about 10 times between 2009 and 2011 in Gaza City. The men met as part of an effort to broker a prisoner swap between Israel and Hamas.

“He was the master of the data on the prisoners,” Mr. Conrad said of Mr. Issa. “He had all the names to be negotiated on.”

Mr. Conrad, however, said it was apparent at the time that Mr. Issa was a subordinate to Mr. al-Jabari. “He was a kind of chief of staff,” he said.

Mr. Issa’s prominence grew only after Mr. al-Jabari’s assassination, but he still was keen to stay out of view. Few images of Mr. Issa are in the public domain.

Mr. Awawdeh, the analyst, called Mr. Issa a man who liked to “remain in the shadows” and who seldom granted interviews to the media.

In one of those rare interviews , Mr. Issa spoke in 2021 about his role in the indirect talks that resulted in Israel exchanging more than 1,000 Palestinian prisoners for a single Israeli soldier, Sgt. First Class Gilad Shalit, and his hopes for a future conflict with Israel.

“Even if the resistance in Palestine is monitored by the enemy at all hours, it will surprise the enemy,” he told Al Jazeera at the time.

In a separate interview with a Hamas publication in 2005, Mr. Issa lauded militants who raided Israeli settlements and military bases, calling the actions “heroic” and an “advanced activity.”

What is known about his early life?

Mr. Issa was born in the Bureij area of central Gaza in 1965, but his family hails from what is now the Ashkelon area in Israel.

A Hamas member for decades, he was involved with the militant group’s effort of pursuing Palestinians who were believed to have collaborated with Israel, according to Mr. Awawdeh.

Mr. Issa spent time in prisons operated by both Israel and the Palestinian Authority.

Rear Adm. Daniel Hagari, a spokesman for the Israeli military, has said that Mr. Issa helped plan the Hamas-led Oct. 7 attack. Mr. Issa is also thought to have planned operations aimed at infiltrating Israeli settlements during the second intifada in the 2000s, Mr. Milshtein said.

An earlier version of this article misstated the surname of a former Israeli military intelligence chief. He is Tamir Hayman, not Heyman.

How we handle corrections

The United States and Britain impose sanctions on a Gazan news outlet over ties to Hamas.

The United States and Britain imposed sanctions Wednesday on Gaza Now, a news organization based in the enclave that the allies accused of raising money for Hamas and helping to finance its terrorist activities.

The Treasury Department said that Gaza Now started a fund-raising effort in support of Hamas following its attack on Israel in October. The measures also target the founder of Gaza Now and two companies that donated thousands of dollars to the news outlet.

The sanctions are the latest attempt to disrupt the flow of funds to Hamas since the Oct. 7 attack. The United States estimates that Hamas controls $500 million worth of assets that it uses to finance terrorism, and the Biden administration has been working with American allies to crack down on sanctions evasion.

“Treasury remains committed to degrading Hamas’ ability to finance its terrorist activities, including through online fund-raising campaigns that seek to funnel money directly to the group,” Brian E. Nelson, the Treasury’s under secretary for terrorism and financial intelligence, said in a statement.

The sanctions freeze any Gaza Now assets held in the United States or Britain and cut its backers off from much of the global financial system.

Gaza Now operates a website and broadcasts television coverage and commentary over a satellite channel and on social media platforms.

Media organizations are not common targets of U.S. sanctions, but the Treasury Department did in 2022 impose financial restrictions on Russian outlets that were spreading disinformation.

Although the Biden administration has been trying to curtail the finances of Hamas, it has also been using sanctions to curb violence by Israelis accused of attacks on Palestinians in the West Bank.

— Alan Rappeport Reporting from Washington

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  1. TRIP Services

    The TRIP laboratory (Translational Research Initiatives in Pathology) offers high quality services in cellular, molecular, quantitative and computational pathology to the University of Wisconsin research community and beyond. We offer services in the areas of: histology, tissue microarray (TMA) creation, immunohistochemistry (IHC - IF), in situ hybridization, imaging of slides (RGB and ...

  2. About TRIP

    Our mission at TRIP (Translational Research Initiatives in Pathology) is to provide high quality services in cellular, molecular, quantitative and computational pathology to the UW campus research community and beyond. We offer histological, histochemical, spatial and molecular profiling and imaging analyses services such as tissue microarray (TMA) creation, imaging of slides in RGB and ...

  3. Translational Research Initiatives in Pathology (TRIP)

    The TRIP lab is providing services complying with COVID-19 campus and CDC guidelines. ... (TRIP) Lab provides technical and consultative support to the UW translational and collaborative research community; and partners with the UW Carbone Cancer Center Biobank as part of Translational Science BioCore (TSB). On a fee-for-service basis, TRIP ...

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    TSB - TRIP Lab. The TSB Translational Research Initiatives in Pathology (TRIP) laboratory provides specialized histological, histochemical, molecular and imaging analyses and consultative services to support the individual research needs of investigators within the UW campus and beyond.

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    The TRIP Lab is offering tissue microarray (TMA) creation services with its brand new TMA Grand Master arrayer. This automated instrument can easily and quickly create TMA blocks with great precision and also extract paraffin embedded tissue samples to standard 0.2 ml PCR tubes to be used later in various molecular pathology applications. TMAs ...

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    TRIP LAB. The TRIP Lab provides routine histology, including paraffin and frozen processing, embedding, and sectioning. To submit a service request, please set up an account with iLab. They also do routine and some special histological staining. Please inquire at [email protected] for the stains. The histopathology services of the UW TRIP ...

  8. Going Spatial: New transcriptomics technology arrives at UW-Madison

    Images by Dr. Karla Esbonda, UW TRIP LAB. Spatial transcriptomics relates single-cell transcriptomes to the spatial organization of those cells in organs and tissue slices. Dr. Tyler Duellman, an assistant researcher at UWBC, explains, "Until recently, there has been a technological barrier for the study of transcriptomal dynamics within a ...

  9. UW-Madison

    TRIP Lab. Looking for Translational Research in Pathology? Leadership. Department of Pathology and Laboratory Medicine Leadership. ... Department of Pathology and Laboratory Medicine 3170 UW Medical Foundation Centennial Building (MFCB) 1685 Highland Avenue Madison, WI 53705-2281; Email: [email protected]

  10. 10X Genomics Visium Spatial Gene Expression

    The Gene Expression Center offers the Visium Spatial Gene Expression Assay in collaboration with the UW-Madison TRIP lab, which provides the imaging service. Libraries are prepared in the GEC and sequenced on the NovaSeq in collaboration with the UWBC DNA Sequencing Facility followed by optional analysis by the UWBC Bioinformatics Resource Center .

  11. UW Translational Research Initiatives in Pathology (TRIPath)

    The UW Translational Research Initiatives in Pathology (TRIPath) lab provides histology, TMA creation, IHC & ISH, imaging, image analysis, cell line authentication, molecular, control tissue & TMA repository, and spatial & molecular profiling services.

  12. Microscopy at UW-Madison. We use. We know. We innovate

    Translational Research Initiatives in Pathology (TRIP) Lab TRIP provides high quality services in cellular, molecular, quantitative and computational pathology to the UW campus research community and beyond. TRIP offers histological, histochemical, molecular and imaging analyses services such as tissue microarray (TMA) creation, imaging of ...

  13. TRIP Study

    Research Design. TRIP was a descriptive longitudinal study with four age- and gender-stratified groups: 100 adult PLWHA in Northeast Ohio; 50 adults 18-50 years of age; 50 adults > 51 years of age. All subjects completed baseline assessments of stress (psychometric scales, heart rate variability, and serum biomarkers) and isolation, as well as ...

  14. UW Laboratory Test Guide

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  15. Contact Us

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    The UW Office of Global Affairs, in collaboration with the German Center for Innovation & Science, the German Research Foundation, and UW CoMotion, would like you to know about the Falling Walls Lab, a great opportunity for students to share their ideas and solutions for the challenges of our time.. The Falling Walls Lab is coming to Seattle on May 16th!

  18. U.N. Security Council approves Gaza cease-fire resolution : NPR

    Israel cancels high-level talks in Washington after cease-fire vote clears the U.N. Updated March 25, 2024 2:43 PM ET Originally published March 25, 2024 11:15 AM ET. By . Jaclyn Diaz ,

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    Phoebe Connelly is The Washington Post's first-ever senior editor for AI strategy and innovation. Appointed to the role in February, Connelly joins a wave of dedicated AI editorial and product strategists entering major newsrooms.Outlets including The Wall Street Journal and The New York Times are actively hiring for similar roles.. At the Post, Connelly's job, first and foremost, is to ...

  24. Service Request

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  26. Forget rest stops. Plan your road trip around playgrounds

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  27. Middle East Crisis: U.S. Says Israel Has Agreed to Try to Reschedule

    The U.S. says Israel has agreed to try to reschedule a canceled trip. A 25-year-old was killed in Israel, while 7 were killed overnight in Lebanon. Two days after the U.N. call for a cease-fire ...

  28. Acknowledging TRIP

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  29. Netanyahu cancels Israeli delegation to US over UN Gaza vote

    The high-level delegation was due to travel to Washington to discuss a planned Israeli military operation in the southern Gaza city of Rafah, where more than a million Palestinians are taking shelter.